Electroporation
Electro Cell Fusion
Sonoporation Sonitron
Sonoporation Sonopore
Cell Stretching System
Electro Cell Fusion Publications
ECFG21/LF201/LF101 Cell Fusion Systems
| Nuclear
Transfer Human |
Noggle S et al. Human oocytes reprogram somatic cells to a pluripotent state. Nature. 2011 Oct 5;478(7367):70-5. |
| Nuclear
Transfer Pigs |
Kim GA, Lee EM, Cho B, Alam Z, Kim SJ, Lee S, Oh HJ, Hwang JI, Ahn C, Lee BC Generation by somatic cell nuclear transfer of GGTA1 knockout pigs expressing soluble human TNFRI-Fc and human HO-1. Transgenic Res. 2019 Feb;28(1):91-102. Appeltant R. et al. Faster, cheaper, defined and efficient vitrification for immature porcine oocytes through modification of exposure time, macromolecule source and temperature. Cryobiology. 2018 Sep 13. pii: S0011-2240(18)30210-4. Santos E. et al. The effect of resveratrol on the developmental competence of porcine oocytes vitrified at germinal vesicle stage. Reprod Domest Anim. 2018 Apr;53(2):304-312. Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes. Theriogenology. 2018 Mar 1;108:29-38. Lee J, You J1, Lee GS, Lee ST, Hyun SH, Lee E Combined Treatment with Demecolcine and 6-Dimethylaminopurine during Postactivation Improves Developmental Competence of Somatic Cell Nuclear Transfer Embryos in Pigs. Anim Biotechnol. 2018 Jan 2;29(1):41-49. Lee J, Lee H, Lee Y, Park B, Elahi F, Lee ST, Park CK, Hyun SH, Lee E In vitro oocyte maturation in a medium containing reduced sodium chloride improves the developmental competence of pig oocytes after parthenogenesis and somatic cell nuclear transfer. Reprod Fertil Dev. 2017 Aug;29(8):1625-1634. Dang-Nguyen TQ. et al. Improvement of the developmental competence of porcine oocytes collected from early antral follicles by cytoplast fusion. J Reprod Dev. 2017 Feb 16;63(1):59-65. Shimatsu Y. et al. Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer. Exp Anim. 2016;65(1):37-43. Jeon Y, Nam YH, Cheong SA, Kwak SS, Lee E, Hyun SH. Absence of nucleolus formation in raccoon dog-porcine interspecies somatic cell nuclear transfer embryos results in embryonic developmental failure. J Reprod Dev. 2016 Aug 25;62(4):345-50. Hyun H, Lee SE, Son YJ, Shin MY, Park YG, Kim EY, Park SP Cell Synchronization by Rapamycin Improves the Developmental Competence of Porcine SCNT Embryos. Cell Reprogram. 2016 Jun;18(3):195-205. Kurome M, et al. Nuclear transfer and transgenesis in the pig. Methods Mol Biol. 2015;1222:37-59. Lee J, You J, Lee GS, Hyun SH, Lee E. Pig oocytes with a large perivitelline space matured in vitro show greater developmental competence after parthenogenesis and somatic cell nuclear transfer. Mol Reprod Dev. 2013 Sep;80(9):753-62. Shimatsu Y. et al. Production of cloned NIBS (Nippon Institute for Biological Science) and α-1, 3-galactosyltransferase knockout MGH miniature pigs by somatic cell nuclear transfer using the NIBS breed as surrogates. Xenotransplantation. 2013 Apr 14. Park SJ, Park HJ, Koo OJ, Choi WJ, Moon JH, Kwon DK, Kang JT, Kim S, Choi JY, Jang G, Lee BC Oxamflatin improves developmental competence of porcine somatic cell nuclear transfer embryos. Cell Reprogram. 2012 Oct;14(5):398-406. Klymiuk N. et al. Production of cloned NIBS (Nippon Institute for Biological Science) and α-1, 3-galactosyltransferase knockout MGH miniature pigs by somatic cell nuclear transfer using the NIBS breed as surrogates. FASEB J. 2012 Mar;26(3):1086-99. Jeon Y, Jeong SH, Biswas D, Jung EM, Jeung EB, Lee ES, Hyun SH. Cleavage pattern and survivin expression in porcine embryos by somatic cell nuclear transfer. Theriogenology. 2011 Oct 15;76(7):1187-96. Biswas D, Jung EM, Jeung EB, Hyun SH. Effects of vascular endothelial growth factor on porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer. Theriogenology. 2011 Jan 15;75(2):256-67. Biswas D, Jung EM, Jeung EB, Hyun SH. Enhancement of cytoplasmic maturation of in vitro-matured pig oocytes by mechanical vibration. J Reprod Dev. 2010 Apr;56(2):285-90. Mizobe Y. et al. Enhancement of cytoplasmic maturation of in vitro-matured pig oocytes by mechanical vibration. J Reprod Dev. 2010 Apr;56(2):285-90. Himaki T. et al. Latrunculin A dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells. Cell Reprogram. 2010 Apr;12(2):127-31. Miyoshi K. et al. Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos. Cell Reprogram. 2010 Feb;12(1):67-74. Song K, Hyun SH, Shin T, Lee E. Post-activation treatment with demecolcine improves development of somatic cell nuclear transfer embryos in pigs by modifying the remodeling of donor nuclei. Mol Reprod Dev. 2009 Jul;76(7):611-9. Fujimura T et al. Effects of recloning on the efficiency of production of alpha 1,3-galactosyltransferase knockout pigs Journal of Reproduction and Development, Volume 54, Number 21, Pages 58-62, February 2008 Miyoshi K et al. Birth of cloned miniature pigs derived from somatic cell nuclear transferred embryos activated by ultrasound treatment Molecular Reproduction and Development, Volume 74 Issue 12, Pages 1568-1574, December 2007 Song K et al. Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos Journal of Veterinary Science, Volume 8, Number 1, Pages 81-87, March 2007 Miyoshi K et al. In Vitro Development of Cloned Embryos Derived from Miniature Pig Somatic Cells after Activation by Ultrasound Stimulation Cloning Stem Cells, Volume 8, Number 3, Pages 159-165, 1 September 2006 Miyoshi K et al. Optimization of Ca2+ Concentrations in Fusion and Activation Media for Production of Cloned Embryos from Miniature Pig Somatic Cells Journal of Reproduction and Development, Volume 51, Number 6, Pages 699-706, December 2005 Kawahara M et al. Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promoting factor activity during nuclear transfer Reproduction, Volume 130, Issue 3, Pages 351-357, September 2005 Miyoshi K et al. Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig Biology of Reproduction, Volume 67, Issue 2, Pages 540-545, August 2002 Miyoshi K et al. Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein-free medium BMC Developmental Biology, Volume 1, Issue 12, 11 July 2001 Miyoshi K et al. Establishment of a Porcine Cell Line from In Vitro-Produced Blastocysts and Transfer of the Cells into Enucleated Oocytes Biology of Reproduction, Volume 62, Issue 6, Pages 1640-1646, June 2000 |
| Nuclear
Transfer Bovine |
Park MJ, Kim EY, Kang MJ, Lee JB, Jeong CJ, Park SP Investigation of the Developmental Potential and Developmental Kinetics of Bovine Parthenogenetic and Somatic Cell Nuclear Transfer Embryos Using a Time-Lapse Monitoring System. Cell Reprogram. 2017 Aug;19(4):245-254. Park MJ, Lee SE, Kim EY, Lee JB, Jeong CJ, Park SP Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer. Cell Reprogram. 2015 Jun;17(3):199-210. Min SH, Lee E, Son HH, Yeon JY, Koo DB. Forced collapse of the blastocoel enhances survival of cryotop vitrified bovine hatching/hatched blastocysts derived from in vitro fertilization and somatic cell nuclear transfer. Cryobiology. 2013 Apr;66(2):195-9. Kim EY, Park MJ, Park HY, Noh EJ, Noh EH, Park KS, Lee JB, Jeong CJ, Riu KZ, Park SP. Improved cloning efficiency and developmental potential in bovine somatic cell nuclear transfer with the oosight imaging system. Cell Reprogram. 2012 Aug;14(4):305-11. Chacón L. et al. Effect of cryopreservation and in vitro culture of bovine fibroblasts on histone acetylation levels and in vitro development of hand-made cloned embryos. Zygote. 2011 Aug;19(3):255-64 Wongsrikeao P, Sutou S, Kunishi M, Dong YJ, Bai X, Otoi T. Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter. Prion. 2011 Jan-Mar;5(1):39-46. Chacón L. et al. Production of bovine cloned embryos with donor cells frozen at a slow cooling rate in a conventional freezer (-20 degrees C). Zygote. 2009 Nov;17(4):341-51. Nel-Themaatd et al. Cloned Embryos from Semen. Part 2: Intergeneric Nuclear Transfer of Semen-Derived Eland (Taurotragus oryx) Epithelial Cells into Bovine Oocytes Cloning Stem Cells, Volume 10, Number 1, Pages 161-172, 1 March 2008 Taniguchi M et al. Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution Journal of Reproduction and Development, 10 April 2007 Patel OV et al. Expression of trophoblast cell-specific pregnancy related genes in somatic-cell cloned bovine pregnancies Biology of Reproduction, Volume 70, Issue 4, Pages 1114-1120, April 2004 Arat S et al. In Vitro Development of Bovine Nuclear Transfer Embryos from Transgenic Clonal Lines of Adult and Fetal Fibroblast Cells of the Same Genotype Biology of Reproduction, Volume 66, Issue 6, Pages 1768-1774, June 2002 Gibbons J et al. Enhanced Survivability of Cloned Calves Derived from Roscovitine-Treated Adult Somatic Cells Biology of Reproduction, Volume 66, Issue 4, Pages 895-900, April 2002 |
| Nuclear
Transfer Dogs |
Jeon Y, Nam YH, Cheong SA, Kwak SS, Lee E, Hyun SH. Absence of nucleolus formation in raccoon dog-porcine interspecies somatic cell nuclear transfer embryos results in embryonic developmental failure. J Reprod Dev. 2016 Aug 25;62(4):345-50. Oh HJ, Choi J, Kim MJ, Kim GA, Jo YK, Choi YB, Lee BC. Propagation of elite rescue dogs by somatic cell nuclear transfer. Anim Sci J. 2016 Jan;87(1):21-6. Park J, Oh H, Hong S, Kim M, Kim G, Koo O, Kang S, Jang G, Lee B Effective donor cell fusion conditions for production of cloned dogs by somatic cell nuclear transfer. Theriogenology. 2011 Mar 1;75(4):777-82. |
| Nuclear
Transfer Cats |
Gómez MC. et al. Trichostatin A modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability. Cell Reprogram. 2011 Aug;13(4):315-29. Gómez MC. et al. Generation of domestic transgenic cloned kittens using lentivirus vectors. Cloning Stem Cells. 2009 Mar;11(1):167-76. Yin XJ1, Lee HS, Yu XF, Kim LH, Shin HD, Cho SJ, Choi EG, Kong IK Production of second-generation cloned cats by somatic cell nuclear transfer. Theriogenology. 2008 May;69(8):1001-6. Gómez MC. et al. Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells. Cloning Stem Cells. 2008 Dec;10(4):469-83. Yin XJ et al. Generation of Cloned Transgenic Cats Expressing Red Fluorescence Protein Biology of Reproduction, Volume 78, Issue 3, Pages 425-431, March 2008 Gomez MC et al. Chromosomal Aneuploidy in African Wildcat Somatic Cells and Cloned Embryos Cloning Stem Cells, Volume 8, Number 2, Pages 69-78, 1 June 2006 Gomez MC et al. Birth of African Wildcat Cloned Kittens Born from Domestic Cats Cloning and Stem Cells, Volume 6, Number 3, Pages 247-258, 1 September 2004 Gomez MC et al. Nuclear Transfer of Synchronized African Wild Cat Somatic Cells into Enucleated Domestic Cat Oocytes Biology of Reproduction, Volume 69, Issue 3, Pages 1032-1041, September 2003 |
| Nuclear
Transfer Boars |
Williams NE et al. A Comparison of Reproductive Characteristics of Boars Generated by Somatic Cell Nuclear Transfer to Highly Related Conventionally Produced Boars Cloning Stem Cells, Volume 8, Number 3, Pages 130-139, 1 September 2006 |
| 2 Cell
Embryos (tetraploid) |
Park MR. et al. Chromosome remodeling and differentiation of tetraploid embryos during preimplantation development. Dev Dyn. 2011 Jul;240(7):1660-9. Tanimoto Y et al. Embryonic stem cells derived from C57BL/6J and C57BL/6N mice. Comp Med. 2008 Aug;58(4):347-52 Amano T et al. Nuclear transfer reprogramming does not improve the low developmental potency of embryonic stem cells induced by long-term culture Reproduction, Volume 132, Issue 2, Pages 257-263, August 2006 Hochedlinger K et al. Ectopic Expression of Oct-4 Blocks Progenitor-Cell Differentiation and Causes Dysplasia in Epithelial Tissues Cell, Volume 121, Issue 3, Pages 465-477, 6 May 2005 Wang Z et al. At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation Developmental Biology, Volume 275, Issue 1, Pages 192-201, 1 November 2004 Hochedlinger K et al. Reprogramming of a melanoma genome by nuclear transplantation Genes & Development, Volume18, Issue15, Pages 1875-1885, 1 August 2004 Suzuki H et al. Electrofusion of Blastomeres of Hamster 2-Cell Embryos and Dynamic Changes of the Cytoskeletal Distribution Journal of Reproduction and Development, Volume 47, Number 4, Pages 227-235, August 2001 |
| Oocyte Activarion |
Kaneko H, Kikuchi K, Men NT, Noguchi J Embryo production by intracytoplasmic injection of sperm retrieved from Meishan neonatal testicular tissue cryopreserved and grafted into nude mice. Anim Sci J. 2019 Feb;90(2):158-166. Nakano K, Watanabe M, Matsunari H, Matsuda T, Honda K, Maehara M, Kanai T, Hayashida G, Kobayashi M, Kuramoto M, Arai Y, Umeyama K, Fujishiro SH, Mizukami Y, Nagaya M, Hanazono Y, Nagashima H. Generating porcine chimeras using inner cell mass cells and parthenogenetic preimplantation embryos. PLoS One. 2013 Apr 23;8(4):e61900. Maehara M, Matsunari H, Honda K, Nakano K, Takeuchi Y, Kanai T, Matsuda T, Matsumura Y, Hagiwara Y, Sasayama N, Shirasu A, Takahashi M, Watanabe M, Umeyama K, Hanazono Y, Nagashima H Hollow fiber vitrification provides a novel method for cryopreserving in vitro maturation/fertilization-derived porcine embryos. Biol Reprod. 2012 Dec 6;87(6):133. Matsunari H, Maehara M, Nakano K, Ikezawa Y, Hagiwara Y, Sasayama N, Shirasu A, Ohta H, Takahashi M, Nagashima H. Hollow fiber vitrification: a novel method for vitrifying multiple embryos in a single device. J Reprod Dev. 2012;58(5):599-608. Egashira A. et al. A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation. Fertil Steril. 2009 Dec;92(6):2037.e5-9. Kanatsu-Shinohara M. et al. Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells. Biol Reprod. 2008 Dec;79(6):1121-8. Hirabayashi M. et al. Factors affecting full-term development of rat oocytes microinjected with fresh or cryopreserved round spermatids. Exp Anim. 2008 Jul;57(4):401-5. |
| Chromosomal Analysis |
Watanabe H et al. A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially J Assist Reprod Genet. 2010 Sep;27(9-10):581-8. |
| Mammalian
Cell Fusion |
Youjia Guo, Atsushi Kawaguchi, Masaru Takeshita, Takeshi Sekiya, Mikako Hirohama, Akio Yamashita, Haruhiko Siomi, Kensaku Murano Potent mouse monoclonal antibodies that block SARS-CoV-2 infection J Biol Chem. 2021 Jan 29;100346. Tsutsumiuchi M, Hoshino H, Kogami A, Tsutsumiuchi T, Yokoyama O, Akama TO, Kobayashi M Preferential expression of sialyl 6'-sulfo N-acetyllactosamine-capped O-glycans on high endothelial venules in human peripheral lymph nodes. Lab Invest. 2019 Oct;99(10):1428-1441. Wang Y, Dzakah EE, Kang Y, Cai Y, Wu P, Cui Y, Huang Y, He X Development of anti-Müllerian hormone immunoassay based on biolayer interferometry technology. Anal Bioanal Chem. 2019 Aug;411(21):5499-5507. Gong B. et al. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities. N Biotechnol. 2016 Sep 25;33(5 Pt A):551-64. Kang K, Wu P, Li W, Tang S, Wang J, Luo X, Xie M Evaluation of a newly developed quantitative heart-type fatty acid binding protein assay based on fluorescence immunochromatography using specific monoclonal antibodies. Scand J Clin Lab Invest. 2015;75(8):693-8. Iinuma H et al. Superior Protective and Therapeutic Effects of IL-12 and IL-18 Gene-Transduced Dendritic Neuroblastoma Fusion Cells on Liver Metastasis of Murine Neuroblastoma J Immunol. 2006 Mar 15;176(6):3461-9. |
| Plant
Cell Fusion |
Md Hassanur Rahman, Erika Toda, Takashi Okamoto In Vitro Production of Zygotes by Electrofusion of Rice Gametes Methods Mol Biol. 2020;2122:257-267. Toda E, Koiso N, Takebayashi A, Ichikawa M, Kiba T, Osakabe K, Osakabe Y, Sakakibara H, Kato N, Okamoto T An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice. Nat Plants. 2019 Apr;5(4):363-368. Xu D, Pan L, Zhao H, Zhao M, Sun J, Liu D Breeding and identification of novel koji molds with high activity of acid protease by genome recombination between Aspergillus oryzae and Aspergillus niger. J Ind Microbiol Biotechnol. 2011 Sep;38(9):1255-65. |
| Fusion of Giant Unilamellar Vesicles, Droplets, Liposome, etc. |
Miyakawa S. et al. A Closed System for Pico-Liter Order Substance Transport from a Giant Liposome to a Cell. Micromachines (Basel). 2018 Jul 2;9(7). Saito AC, Ogura T, Fujiwara K, Murata S, Nomura SM Introducing micrometer-sized artificial objects into live cells: a method for cell-giant unilamellar vesicle electrofusion. PLoS One. 2014 Sep 17;9(9):e106853. Terasawa H et al. Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules. Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):5942-7. Tan W et al. Timing controllable electrofusion device for aqueous droplet-based microreactors Lab on a chip, Volume 6, Issue 6, Pages 757-763, June 2006 Tresset G et al. Utilization of Cell-Sized Lipid Containers for Nanostructure and Macromolecule Handling in Microfabricated Devices Analytical. Chemistry, Volume 77, Issue 9, May 2005 Tresset G et al. A Microfluidic Device for Electrofusion of Biological Membranes Seisan Kenkyu, Volume 56, Number 1, Pages 101-104, 2004 |
