Transfection/Electroporation
NEPA21 Super Electroporator
ELEPO21 High Energy Electroporator
NEPA Porator
Cuvette Electroporation Applications
Adherent Electroporation Applications
In Vivo/In Utero/In Ovo/Ex Vivo
Electroporation Applications
CUY21 SC Electroporator
CUY21 EDIT Electroporator
Cuvettes/Chambers/Stand Holders
Cell-Culture-Plate Electrode
In Vivo/In Utero/In Ovo/Ex Vivo
Electrodes
Monitoring System
Accessories
Transfection/Sonoporation
Transfection/Microinjection
Electro Cell Fusion
Single-Cell/Micro-Particle Transfer
Programmable Freezer
Mechanical Vibration
NEPA Porator
Ohm Checkable Exponential Decay Wave Electroporator
Transformation/Transfection Applications
- E. coli
- Bacterial cells
- Yeasts
- Fungi
- Mammalian cells
*The NEPA Porator cannot be used for adherent electroporation (using cell-culture electrodes) or in vivo/in utero/in ovo/ex vivo electroporations.
Ohm check for controlling electroporation
Electroporation is generated by delivering an electric pulse(s), and the delivery of the electric pulse(s) at the same voltage and the same current with the same resistance under the ohm's law is essential for achieving highly reproducible results.
The NEPA Porator can measure the electric impedance (that is almost equal to resistance) of the sample between the electrodes. Checking the impedance prior to electroporation is greatly helpful for avoiding inconstant electroporation, reviewing electroporation results and performing expected electroporation that will bring desired transformation results.
| Voltage setting; 500 V Checked sample impedance: 36 ohms |
Voltage setting; 500 V Checked sample impedance: 3,909 ohms |
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| Output electric pulse measured by oscilloscope |
Output electric pulse measured by oscilloscope |
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Specifications
Settings of exponential decay wave pulse
| Voltage | 100–500 V (5 V resolutionn) 500–3,000 V (100 V resolution) |
| Pulse length | None setting or 1.0-5.0 msec (0.5 msec resolution) |
| Number of pulses | 1 or 2 |
| NEPA Porator exponential decay wave pulse *Pulse length setting: none |
NEPA Porator exponential decay wave pulse *Pulse length setting: 1.0-5.0 msec |
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Measurements of output puless
| Voltage | 100–3,000 V (1 V resolution) |
| Time constant | None setting: Measured value is displayed 1.0-5.0 msec setting: Set pulse length value is displayed |
| Number of pulses | 1 or 2 |
Others
| Impedance Measurement | 15–9,999 Ω (1 Ω resolution) 10,000–50,000 Ω (10 Ω resolution) |
| Save | 99 programs |
| Dimensions | 335 (W) x 205 (D) x 85 (H) mm |
| Weight | 3.2 kg |
*This instrument complies with CE marking, UL standard and CSA standard.
**All features and specifications subject to change without notice.
