Transfection/Electroporation
Transfection/Sonoporation
Transfection/Microinjection
Electro Cell Fusion
ECFG21 Cell Fusion Generator
Applications
LF201 Cell Fusion Generator
LF101 Cell Fusion Generator
Cell Fusion Electrodes
Monitoring System
Accessories
Single-Cell/Micro-Particle Transfer
Programmable Freezer
Cell Stretching System
Mechanical Vibration
LF101 Electro Cell Fusion Generator
Somatic-Cell Nuclear Transfer (SCNT)/Hybridoma Production
LF101 Applications
- Somatic-Cell Nuclear Transfer (SCNT) for Bovine, Pigs, Mice, Rabbits, etc.
- Hybridoma Production for Monoclonal Antibodies
- Plant Protoplast Fusion
- Egg Activation
LF101 Features
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Specialized setting for bovine, pig, mouse and rabbit cloning (somatic cell and embryo nuclear transplant) will result in extremely high fusion probability (for example: more than 90% for bovine cloning). |
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Voltage setting with 1V resolution is possible in the specialized setting for mammalian cloning. It is suitable to output precisely the square pulse wave shape and pulse range as preset and accordingly brings about a higher rate of cell fusion probability. |
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Post fusion function for damage recovery of the cell can be operated with On/Off switching. |
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Specialized setting for plant protoplast and mammalian cell fusion can provide a desirable condition for the cell fusion, which is done with DC pulse (100V, max.) of the precisely controlled voltage and pulse range after producing the pearl chains with DC. It can elevate the rate of hybridoma production (cell fusion) probability. |
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Operation of the device is very easy because both of automatic and manual (number of pulses: 1-99) transmitting of the pulse are possible. And the pulse interval can be selected to set between 0.1-9.9 seconds. The device can keep a constant operating condition anytime. |
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NEPA GENE provides a variety of electrodes for combination use with the device, which is designed to match well with its various settings through our long accumulated experiences. Combination use of the electrodes with the device enables very easy and efficient gene transfection and cell fusion. |
LF101 Specifications
AC Pulse
| Wave Shape | Sine Wave |
| Voltage | 0 - 50V (VP-P) |
| Frequency | 0.1 - 3.9MHz Variable |
| Duration | 0 - 99s |
| Post Fusion | 0～9sec （On・Offスイッチ） |
| Shunt Resistor | Greater than 50 ohm |
DC Pulse
| Wave Shape | Square |
| Voltage | 0 - 999V
0 - 200V (1V resolution) |
| Pulse Length Range | 5～99μs （1μs resolution） |
| Pulse interval | 0.1～9.9s |
| Number of Pulses | 0 - 99 Automatic or Manual |
| Power | 100V （50/60Hz） 2A |
| Size / Weight | 375W×430D×160H mm , 12.7ｋｇ |
All features and specifications subject to change without notice.
