Transfection/Electroporation
Transfection/Sonoporation
Transfection/Microinjection
Electro Cell Fusion
ECFG21 Cell Fusion Generator
Applications
LF201 Cell Fusion Generator
LF101 Cell Fusion Generator
Cell Fusion Electrodes
Monitoring System
Accessories
Single-Cell/Micro-Particle Transfer
Programmable Freezer
Mechanical Vibration
LF201 Electro Cell Fusion Generator
Hybridoma Production/Somatic-Cell Nuclear Transfer (SCNT)
LF201 Applications
- Fusion of Mammalian Cells
- hybridoma production for monoclonal antibodies, immuno-therapy, etc.
- Fusion of Somatic Cell and Egg Cell or Embryonic Cell and Egg Cell
- somatic cell nuclear transfer (SCNT) for animal cloning
- Fusino of 2-Cell Blastomeres
- tetraploid embryos
- Fusion of Plant Cells/Yeasts
- Oocyte Activation
LF201 Features
- Electric Impedance Measurement
| The LF201 easily enables you to measure electric impedance between electrodes. It makes it possible to find an abnormality before electrofusion start. This greatly enhances reproducibility. |
- Accurate Square Wave Pulses
| The LF201 is designed to output precise square wave pulses. The accurate square wave pulses bring high efficiency and renproducibility. Especially an accurate voltage output by 1V resolution setting is important for successful SCNT experiments. |
- Short AC/DC Switching Time
| Switching time from alternate current (AC) ouput to direct current (DC) pulse output is less than 5 microseconds. The AC is for dielectrophoretic alignment of cells (Pearl Chain). After the cell alignment, DC square pulses are output for fusion. Then AC is re-applied for keeping cells together for the rounding off process. The shorter AC/DC switching time leads to an effective fusion. |
- A Variety of CUY Series Electrodes
| The LF201 can be used with a variety of electrodes which make it possible to do a wide range of cell fusion experiments. And custom-made electrodes are easily ordered and available for this LF201 Electro Cell Fusion Instrument. It enables you to realize the experiments which you have actually desired. |
- Two Kinds of DC Pulses
| The LF201 offers two DC pulse modes. A positive (+) square wave only or a bipolar (+/-) square wave. |
LF201 Specifications
AC
| Wave Shape | Sine Wave |
| Voltage | 0-75V (Peak to Peak), 1V resolution |
| Frequency | 1MHｚ |
| Duration | 0-100s (1sec resolution) |
| Post Fusion AC | 0-10s (Sine or Fade Wave) |
| Shunt Resistor | Greater than 50ohm |
DC Pulse
| Wave Shape | Positive
(+) Square Wave Pulse or Bipolar (+/-) Square Wave Pulse |
| Voltage | 0-1200V (1V resolution) |
| Pulse Length | 0-100μs (1μsec resolution) |
| Pulse Interval | 0.1-10s (0.1 sec resolution) |
| Number of Pulses | 1-100 (Automatic/Manual) |
| Resistance Measurement | Up to 39.99kohm |
| AC/DC Switching Time | Less than 5μs |
| Memory | 99 protocols |
| System Requirement | Windows 2000 and Windows XP |
| Power | 100V-240V |
| Dimensions, Weight | 375W x 360D x 170H mm, 9ｋｇ |
All features and specifications subject to change without notice.
