Transfection/Electroporation
NEPA21 Super Electroporator
ELEPO21 High Energy Electroporator
NEPA Porator
Cuvette Electroporation Applications
Adherent Electroporation Applications
In Vivo/In Utero/In Ovo/Ex Vivo
Electroporation Applications
CUY21 SC Electroporator
CUY21 EDIT Electroporator
Cuvettes/Chambers/Stand Holders
Cell-Culture-Plate Electrode
In Vivo/In Utero/In Ovo/Ex Vivo
Electrodes
Monitoring System
Accessories
Transfection/Sonoporation
Transfection/Microinjection
Electro Cell Fusion
Single-Cell/Micro-Particle Transfer
Programmable Freezer
Mechanical Vibration
CUY21EDIT Square Wave Electroporator
In Vivo Transfection System
CUY21EDIT Applications
- In Vivo Transfection
- into mouse/rat brain, eye, muscle, skin, liver, kidney, testis, etc.
- with in vivo electrodes
- In Utero Transfection
- into mouse/rat embryos: cerebral cortex, hippocampus, spinal cord, etc.
- with in utero electrodes
- In Ovo Transfection
- into chick embryos: neural tube, limb bud, digestive organ, etc.
- with in ovo electrodes
- Ex-Vivo Transfection
- into explants, brain slices, organs, whole embryos, etc.
- with ex vivo electrodes
CUY21EDIT Features
- Automated Current Measurement
| Electric current is the major factor to obtain successful electroporation results. The CUY21EDIT makes it possible to measure and display such current automatically. The output current information remarkably helps you optimize your electroporation experiments. |
- Impedance Measurement
| The CUY21EDIT easily enables you to measure impedance (electric resistance) of the target sample and automatically displays it. The fluctuation in resistance value results in changes in current value and as a result it affects the reproducibility of experiments. In order to deliver the optimal current, you need to know the actual resistance of the target sample before electroporation. Because in vivo resistance is less stable than in vitro in general, the impedance value which is measured prior to in vivo electroporation is the important criteria to determine whether the experiment is going well. |
- Accurate Low Voltage Square Wave Pulses
| The CUY21EDIT is designed to output precise low voltage square wave pulses. The accurate and low voltage square wave pulses bring high transfection efficiency and less damage. The Voltage can be set from 1V to 500V in 1V increment. |
- Wide Applications In Combination With A Variety of CUY Series Electrodes
| The CUY21EDIT can be used with more than 250 NEPA GENE's CUY series electrodes which make it possible to do a wide range of electroporation experiments. And custom-made electrodes are easily ordered and available for this CUY21 electroporator. It enables you to realize the experiments which you have actually desired. |
- Safety Function
| The CUY21EDIT has the safety function. If the current flows beyond the limit, This CUY21 electroporator is shut down automatically. This safety function protects the user from the electrical accidents. This CUY21 electroporator also has other unique safety functions. One is the warning beep prior to the pulse output. The other is that red indicator on the front panel lights up during electroporation. |
- Automatic Voltage Measurement
| Actual output voltage of the CUY21EDIT can be measured after the electroporation is executed. By checking the voltage, it is easy to find out whether programmed voltage of DC pulse is generated. If not, there is something wrong with a device. |
CUY21EDIT Specifications
| DC Pulse Waveform | Square | ||||||||
| Impedance
Measurement (Ohm Check) |
Max. 18 Kohm | ||||||||
| Voltage (Volt) | 1-500V (1V resolution) | ||||||||
| Pulse Length (Pon) | 0.1-999.9ms (0.1ms resolution) | ||||||||
| Pulse Interval (Poff) | 0.1-999.9ms (0.1ms resolution) | ||||||||
| Number of pulses (No.) | 1-99 | ||||||||
| Safety Limit (High Current) |
| ||||||||
| Measured
Output (Automatic) |
Voltage: 1-500V Current: 0.01-4.00A | ||||||||
| Memory | 2 Programs | ||||||||
| Dimensions, Weight | 390W x 450D x 250H mm, 15.4kg | ||||||||
| Power | 115V or 220V, 50/60Hz |
Electrode Selection for the CUY21 Electroporator
| Transfection Targets | Electrodes | |
| Chick and Quail
Embryo (In Ovo) |
Neural Tube
(HH* stage 10) Mesencephalon (HH stage 10) Diencephalon (HH stage 10) |
CUY610
series CUY611 series |
| Chick Embryo (In Ovo) | Optic
Vesicle (HH stage 10) Head Ectoderm (HH stage 10) Lens (HH stage 10) Lateral Plate Mesoderm (LPM) Somitic Mesoderm (HH stage 7-9) Limb Bud (HH stage 7-9) Digestive Organ (HH stage 7-9) Ventricle (HH stage 30) Retina (HH stage 38-42) CUY610 series |
CUY611
series CUY613 series CUY614 & CUY615 |
| Chick
Embryo (Organ Culture) |
Digestive Organ Epithelial Cell (HH stage 27-28) | CUY520P5 |
| Chick
Embryo (New Culture) |
Gastrulation (HH stage 3-7) Neural Plate (HH stage 3-7) |
CUY701 series combination |
| Mouse Embyo (In Utero) | Cerebral
Cortex, All Area (E12.5) Cerebral Cortex, All Area (> E13.5) Cerebral Cortex, Specific Area (> E13.5) Forebrain Ventricle (E14) Spinal Cord (E10.5) |
CUY650P3 CUY650P5, P7 CUY650P0.5-3, P1-5 CUY650P1-5 CUY651P |
| Mouse and
Rat (Whole Embryo Culture) |
Neural Tube
(Mouse: E9.5, Rat: E11.5) Neural Plate (Mouse: E7-13) Metencephalon (Mouse: E7-13) Somite (Mouse: E7-13) Telencephalon (Mouse: E7-13) |
CUY520P15,
P20, P25 CUY650P3, P5, P10 |
| Adult Mouse, Rat, Rabbit and Dog (In Vivo) | Skin (Epidermis, Dermis, Hypodermis) | CUY663 series |
| Adult Mouse, Rat,
Rabbit, Dog and Monkey (DNA Vaccine) |
Posterior
Region of Leg Calf Anterior Tibial Muscle |
CUY560-3,
-5 (Injection) CUY650P3, P5 (Holding) CUY647P10X5 (Holding) |
| Adult Mouse and
Rat (In Vivo) |
Kidney Liver Vessel |
CUY650P5 CUY650P10 CUY250 series combination |
| Adult Mouse and
Rat (In Vivo) |
Bladder Testis Ovary |
CUY647P5X10 CUY650P5, P10 |
| Adult Mouse and
Rat (In Vivo) |
Synovial
Membrane Knee Cartilage |
CUY650P5, P10 |
| Adult Mouse and
Rat (In Vivo) |
Brain | CUY200S *CUY21 SC is recommended. |
| Newborn Mouse and
Rat (In Vivo) |
Retina
(P0-P3) Cornea |
CUY675P3,
P5 CUY670, CUY671P1 |
| Newborn Mouse and
Rat (In Vivo) |
Brain
Ventricle (P0-P3) Brain Ventricle (P4-P8) |
CUY650P3,
P5 CUY650P7 |
| Tissue
Fragment (Ex Vivo) |
Brain Slice, Tissue Slice, Skin Slice | CUY701 series combination |
| Bee (In Vivo) | Brain | CUY567 |
| Silkworm | Egg | CUY495P10 |
| Xenopus
Embryo (In Vivo) |
Stage 12.5 | CUY700P20 & CUY195P0.3 |
| Fish | Egg | CUY535,
CUY536 CUY537P0.3, P0.5 |
| Plant (In Vivo) | Seed Embryo | CUY495
P10 *CUY21EDIT is recommended. |
| Mammalian Adherent
Cell (Single Cell) |
CUY230 *CUY21 SC is used. | |
| Plant
Protoplast (Single cell) |
CUY230 *CUY21 SC is used. | |
* HH: Hamburger Hamilton
We can make special customized electrodes upon your request.
Please feel free to contact us.
All features and specifications subject to change without notice.
